![]() Wang AJ, Ao Q, Wei YJ et al (2007) Physical properties and biocompatibility of a porous chitosan-based fiber-reinforced conduit for nerve regeneration. Seglen PO (1976) Preparation of isolated rat liver cells. Pattison MA, Wurster S, Webster TJ (2005) Three-dimensional, nano-structured PLGA scaffolds for bladder tissue replacement applications. Onodera K, Sakata H, Yonekawa M et al (2006) Artificial liver support at present and in the future. Nelson CM, Tien J (2006) Microstructured extracellular matrices in tissue engineering and development. Maurin B, Canadas P, Baudriller H et al (2008) Mechanical model of cytoskeleton structuration during cell adhesion and spreading. Ki CS, Park SY, Kim HJ et al (2008) Development of 3-D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration. This review will also discuss the applications of cell-electrospun structures in tissue engineering. In this review, the process of cell-electrospinning and the materials used in this process will be discussed. Khang D, Lu J, Yao C et al (2008) The role of nanometer and sub-micron surface features on vascular and bone cell adhesion on titanium. Cell-electrospinning is based on the basic process of electrospinning for producing viable cells encapsulated in the micro/nanofibers. Many approaches have been used such as direct cellular injection onto present vascular beds, micro-carrier attachment and scaffold implants seeded with cells. Jou CH, Chen WC, Yang MC et al (2008) In vitro biocompatibility of three-dimensional chitosan scaffolds immobilized with chondroitin-6-sulfate. Hepatic tissue engineering is focused on creating a whole, implantable and functional liver. Hong Z, Zhang P, He C et al (2005) Nano-composite of poly(L-lactide) and surface grafted hydroxyapatite: mechanical properties and biocompatibility. Hay ED (Ed) (1991) Cell biology of extracellular matrix. Diabetologia 40(5):606–609Ĭhua KN, Lim WS, Zhang PC et al (2005) Stable immobilization of rat hepatocyte spheroids on galactosylated nanofiber scaffold. 10.1038/s41591-z.Berti L, Kellerer M, Capp E et al (1997) Leptin stimulates glucose transport and glycogen synthesis in C2C12 myotubes: evidence for a P13-kinase mediated effect. Response Differences of HepG2 and Primary Mouse Hepatocytes to Morphological Changes in Electrospun PCL Scaffolds. Blended Electrospinning with Human Liver Extracellular Matrix for Engineering New Hepatic Microenvironments. Liver Tissue Engineering as an Emerging Alternative for Liver Disease Treatment. Liver Tissue Engineering: From Implantable Tissue to Whole Organ Engineering. Taken together, our findings provide a reproducible approach and utilization of animal tissue-derived ECM and emphasize the synergism of topographical stimuli and biochemical cues on electrospun scaffolds in liver tissue engineering.ĭecellularized extracellular matrix electrospinning liver tissue engineering scaffolds topography.īritish Liver Trust The Alarming Impact of Liver Disease in the UK. Scanning electron microscopy shows that cell morphology was distinctly different on hybrid scaffolds compared to control groups, where HepG2 began to form a monolayer toward the end of the culture period meanwhile, typical hepatic markers and ECM genes were also influenced, such as an increasing trend of albumin appearing on the hybrid scaffolds. Cell viability and DNA quantification imply steady cell proliferation over the culture period, with the highest albumin secretion observed on the hybrid scaffold. Human hepatocytes (HepG2) were seeded to analyze the scaffold biocompatibility. The results show that our novel hybrid scaffolds have enhanced hydrophilicity, and the nanotopography retained its original form after hydrolytic degradation for 14 days. Water contact angle, tensile test, and degradation studies were conducted to analyze scaffold hydrophilicity, mechanical properties, and stability. In this study, we investigate both of these synergistic effects and developed a new procedure to directly blend whole-organ vascular perfusion-decellularized rat liver ECM (dECM) into electrospun fibers with tailored surface nanotopography. The separate employment of topographic or biological cues on a scaffold has both shown influences on hepatocyte survival and growth. The formulation of a multifunctional scaffold that has the potential to mimic the complex extracellular matrix (ECM) and their influence on cellular behavior, are essential for culturing cells on a construct. Liver tissue engineering aims to reproduce or restore function through in vitro tissue constructs, which may lead to alternative treatments for active and chronic liver disease. Donor liver shortage is a crucial global public health problem as whole-organ transplantation is the only definitive cure for liver disease. ![]()
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